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Unrelatedness of factor VIII‐derived non‐A/non‐B hepatitis and hepatitis B virus
Author(s) -
Fields H. A.,
Davis C. L.,
Margolis H. S.,
McCaustland K. A.,
Wheeler C. M.,
Maynard J. E.,
Bradley D. W.,
Bernlnger M.,
Hammer M.,
Nath N.
Publication year - 1983
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890110108
Subject(s) - virology , hepatitis b virus , virus , biology , hepadnaviridae , dna , hepatitis , hepatitis b , hepatitis b virus dna polymerase , polymerase chain reaction , microbiology and biotechnology , gene , genetics
A DNA hybridization assay was used to detect hepatitis B virus (HBV)‐specific DNA sequences in extracted sera obtained from chimpanzees infected with HBV, hepatitis A virus (HAV), and a factor VIII‐derived non‐A/non‐B (NANB) agent. The results did not reveal any HBV‐DNA homology with sera obtained from animals infected with HAV or factor VIII‐derived NANB. Sera obtained from two HBV‐infected chimpanzees demonstrated that HBV‐specific DNA could be detected during the acute phase of the disease. In addition, an HBV‐specific DNA‐dependent DNA polymerase assay did not demonstrate any statistically significant activity in 12 of 12 NANB acute‐phase specimens or in 6 of 6 NANB chronic‐phase specimens. These results suggest that the factor VIII‐derived NANB agent is unrelated to HBV.