Premium
Solid‐phase immune electron microscopy (SPIEM) by use of protein A and its application for characterization of selected adenovirus serotypes
Author(s) -
Svensson Lennart,
von Bonsdorff CarlHenrik
Publication year - 1982
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890100404
Subject(s) - virology , electron microscope , serology , serotype , virus , capsid , antibody , immune sera , immune system , biology , chemistry , microbiology and biotechnology , immunology , physics , optics
Staphylococcal protein A was used for the anchoring of specific antibodies for a solid‐phase immune electron microscope method (SPIEM). More IgG adsorbed to grids in the absence of protein A than in its presence. However, the virus trapping efficiency was markedly improved in the presence of protein A. The specificity of the test was evaluated by use of different adenovirus preparations and matching rabbit hyperimmune sera. The degree of serological reaction was evaluated by counting the number of particles attached to the grid. Type specific reactions and inter‐ and intrasubgroup reactions were identified.