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Adsorption of purified human cytomegalovirus and induction of early antigens in different cells
Author(s) -
Einhorn Lena,
Gadler Håkan,
Wahren Britta
Publication year - 1982
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890100402
Subject(s) - antigen , virology , cytomegalovirus , human cytomegalovirus , immunofluorescence , in vitro , biology , virus , bioassay , indirect immunofluorescence , cytomegalovirus infection , microbiology and biotechnology , chemistry , herpesviridae , antibody , immunology , viral disease , biochemistry , genetics
Abstract Different human and nonhuman cells were assayed for their capacity to adsorb human cytomegalovirus (CMV Ad. 169) and to support CMV infection in vitro. The CMV adsorptive capacity was assayed by measuring cell‐bound radioactivity after addition of purified 3 H‐ or 125 I‐labeled CMV or by a bioassay for residual infectious virus in the supernatant fluid. Many of the human and nonhuman cell types adsorbed CMV. Induction of CMV early nuclear antigens in the same cells was assayed by anticomplement immunofluorescence staining of fixed cells 1–3 days after infection. CMV early antigens were induced in the human and nonhuman cells that showed a high degree of CMV adsorption.