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Production of hepatitis b e antibody in epstein‐barr virus‐induced b lymphocyte cell lines
Author(s) -
Furuya Yumiko,
Inoue Masayasu,
Yoshida Yoshiya,
Yoshihara Namiko,
Fakada Mitsuru
Publication year - 1982
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890090407
Subject(s) - virology , lymphoblast , antibody , cell culture , biology , virus , antigen , lymphocyte , epstein–barr virus , microbiology and biotechnology , b cell , immunology , genetics
B lymphocytes separated from anti‐HBe‐positive donors were established as lymphoblastoid cell lines by infection with EBV, but anti‐HBe in the culture supernatant from such lymphoblastoid cell lines could not be detected. The lymphoblastoid cell lines were rosetted with HBe antigen‐coupled SRBC to prepare cells for the production of specific anti‐HBe. Antibody activity in the culture supernatant against rosette forming cells was detected by RIA, ID, and R‐PHI tests during the first 1 to 4 wk, but not after 5 wk. The activity in the supernatant was not destroyed by treatment with 2‐mercaptoethanol, indicating that the antibody might be IgG.