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An electron microscopic demonstration of immune complexes of hepatitis b e‐antigen using colloidal gold as a marker
Author(s) -
Stannard Linda M.,
Lemon Margaret,
Hodgkiss Margaret,
Smuts Heidi
Publication year - 1982
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890090303
Subject(s) - hbsag , hbeag , immune system , antigen , virology , antibody , hepatitis b virus , hepatitis , hepatitis b , immune complex , immunology , medicine , virus
Immune complexes of the hepatitis B e‐antigen (HBeAg) could be labeled and thus visually identified in the electron microscope by using antibody to HBeAg (anti‐HBe) tagged with colloidal gold particles. Circulating immune complexes of HBeAg were detected in sera from patients with acute hepatitis B infections as well as from asymptomatic carriers of hepatitis B surface antigens (HBsAg). Sera positive for rheumatoid factor frequently contained mixed aggregates in which immune complexes of HBsAg were closely bound to immune complexes of HBeAg.