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Properties of soluble dna polymerase from sera of hepatitis b virus carriers
Author(s) -
Mao James C. H.,
Otis Ellen R.,
Mushahwar Isa K.,
Overby Lacy R.
Publication year - 1980
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890060404
Subject(s) - hepatitis b virus dna polymerase , dna polymerase , polymerase , hbsag , microbiology and biotechnology , hepatitis b virus , virology , hbeag , enzyme , primer (cosmetics) , hepadnaviridae , biology , dna , virus , chemistry , biochemistry , organic chemistry
A soluble DNA polymerase was purified 8,000‐fold from hepatitis B surface antigen positive serum. The molecular weight of the enzyme by gel filtration was about 1.60 × 10 5 , the sedimentation coefficient was 5.5S, the apparent Km for dTTP was 4 MM, the optimum pH in the presence of Mg 2+ was 9.2, and the pI was 4.7. The enzyme was found in HBsAg‐positive sera and required an external primer for activity. The properties of the DNA polymerase were different from hepatitis B virus particle enzyme and from vertebrate and bacterial DNA polymerases. The prevalence of this enzyme did not correlate with HBeAg or particle DNA polymerase in HBsAg‐positive sera.