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Non‐A/Non‐B hepatitis in experimentally infected chimpanzees: cross‐challenge and electron microscopic studies
Author(s) -
Bradley Daniel W.,
Maynard James E.,
Cook E. H.,
Ebert James W.,
Grave Me Clifton R.,
Tsiquaye Kwesi N.,
Kessler Harold,
Zuckerman Arie J.,
Miller Mahlon F.,
Ling ChungMei,
Overby Lacy R.
Publication year - 1980
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890060302
Subject(s) - hepatitis , virology , hepatitis b , strain (injury) , biology , factor ix , biochemistry , anatomy
Inoculation of eight chimpanzees with factor VIII, factor IX, or “H” strain plasma resulted in enzymatic and histopathologic evidence of non‐A/non‐B hepatitis in all eight animals. Challenge of two chimpanzees convalescent from factor VIII‐induced disease with either factor IX or “H” strain plasma resulted in non‐A/non‐B hepatitis only in the animal inoculated with factor IX materials. Reciprocal cross‐challenge of a chimpanzee convalescent from factor IX‐induced disease with factor VIII also produced unequivocal enzymatic and histopathologic evidence of non‐A/non‐B hepatitis. Cross‐challenge of a chimpanzee convalescent from “H” strain‐induced non‐A/non‐B hepatitis with factor VIII did not cause a second bout of non‐A/non‐B hepatitis. These findings suggest the factor VIII materials and “H” strain plasma used in these studies share a common etiologic agent (or agents), but that factor VIII and factor IX may contain two distinct agents. Electron microscopic (EM) examination of thin‐sectioned, acute‐phase liver biopsies from all but one of the chimpanzees receiving the primary inocula revealed the presence of abnormal hepatocyte cytoplasmic structures previously shown to be associated with non‐A/non‐B hepatitis. Crystalline structures containing 25 to 30 nm particles were visualized by EM in the cytoplasm of endothelial or Kupffer cells in acute‐phase liver biopsies obtained from three chimpanzees inoculated with either factor VIII materials or “H” strain plasma.