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The separation and analysis of hepatitis B e antigen
Author(s) -
Howard C. R.,
Zuckerman A. J.
Publication year - 1979
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890040408
Subject(s) - isoelectric point , sepharose , radioimmunoassay , chromatography , antigen , size exclusion chromatography , molecular mass , chemistry , hepatitis b , albumin , microbiology and biotechnology , virology , biochemistry , biology , immunology , enzyme
A solid‐phase radioimmunoassay has been used successfully for detecting hepatitis B e antigen in fractionated hepatitis B virus‐containing serum. Ammonium sulphate precipitation followed by gel filtration through a column of Sepharose CL‐6B resulted in two fractions of antigen‐containing material with molecular weights of 220,000 and 130,000. The smaller of these two fractions was found to possess an average isoelectric point of 4.9 and consisted of two major polypeptide species with estimated molecular weights of 66,000 and 17,000 respectively. Affinity chromatography on Blue Sepharose showed that e antigen was not retained under conditions which bound serum albumin. These results are discussed in relation to the immunopathogenesis of hepatitis B.