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Replication of murine cytomegalovirus in murine Y‐1 cells
Author(s) -
Shanley John D.,
Lutwick Larry I.,
Donta Sam T.
Publication year - 1979
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890040404
Subject(s) - virology , biology , cytomegalovirus , viral replication , cell culture , virus , cytopathic effect , cytoplasm , betaherpesvirinae , herpesviridae , microbiology and biotechnology , viral disease , genetics
Murine Y‐1 cells, a continuous epithelial cell line derived from an adrenal adenocarcinoma, are highly susceptible to murine cytomegalovirus (MCMV) infection. Inoculation of these cells with MCMV results in production of infectious virus and is associated with development of both cytopathic changes and the expression of viral‐specific nuclear and cytoplasmic antigens. Examination of the ultrastructure of infected Y‐1 cells reveals MCMV in various stages of replication. Cytomegalovirus replication in this hormone‐responsive epithelial cell line should provide a model system in which virus‐host cell interactions and their effects on cellular metabolism can be studied.