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Cross‐reactive and type‐specific complement‐fixing structures of oriboca virions
Author(s) -
Karabatsos Nick,
Shope Robert E.
Publication year - 1979
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890030302
Subject(s) - infectivity , centrifugation , complement fixation test , sedimentation coefficient , chemistry , virology , hemagglutination , virus , antibody , microbiology and biotechnology , cleavage (geology) , viral envelope , biology , biochemistry , serology , enzyme , paleontology , fracture (geology) , immunology
Characteristics of two components of Oriboca virus were studied after they were separated and semipurified by velocity centrifugation. A component of low infectivity and broad cross‐reactivity in the complement‐fixation (CF) test had a sedimentation coefficient of 6–7S. Infectious Oriboca virions with hemagglutinating (HA) activity had a sedimentation coefficient of 457S. These virions cross‐reacted broadly with Murutucu viral antibody. The crossreactive, virion‐associated component and a type‐specific CF fragment were released from virions by disruption with Nonidet P‐40. These CF fragments separated by sucrose gradient equilibrium centrifugation had densities of 1.245 gm/ml and 1.181 gm/ml, respectively, and were both interpreted to be envelope structures. The type‐specific CF antigen appeared to be related to the viral hemagglutinin in that it blocked hemagglutination‐in‐hibiting (HI) antibody, whereas the cross‐reactive CF fragment did not.