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Detection of HBeAg and anti‐HBe in acute hepatitis B by a sensitive radioimmunoassay
Author(s) -
Frösner Gert G.,
Brodersen Manfred,
Papaevangelou George,
Sugg Udo,
Haas Hans,
Mushahwar Isa K.,
Ling ChungMei,
Overby Lacy R.,
Deinhardt Friedrich
Publication year - 1978
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890030114
Subject(s) - hbeag , medicine , radioimmunoassay , hbsag , hepatitis b , virology , hepatitis , immunology , hepatitis b virus , virus
A solid‐phase radioimmunoassay using anti‐HBe‐coated polysterene beads and iodine‐ 125 ‐labeled anti‐HBe of human origin was developed for the detection of HBeAg. Anti‐HBe could be determined by a blocking test. Both assays were about 500‐fold more sensitive than immunodiffusion. Few nonspecific positive results for HBeAg could be recognized in the anti‐HBe test by increase in cpm over that of the negative control. HBeAg was not found in acute hepatitis A and non A‐non B heptatis or in a control group of accident patients. On admission to the hospital 12 of 48 (25%) acute hepatitis B patients from Greece and 17 of 20 (85%) acute hepatitis B patients from Germany were HBeAg‐positive. All 39 initially HBeAg negative sera were already anti‐HBe positive. Tests of the acute stage and follow‐up sera of the 20 German patients indicated that HBeAg is regularly present in the incubation period and early acute phase of hepatitis B. After onset of disease the antigen is cleared from the serum very rapidly in uncomplicated cases and is usually followed by the appearance of anti‐HBe. Like anti‐HBe, anti‐HBe. can serve as a tool for the diagnosis of hepatitis B after the disappearance of HBsAg.