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Infection of human amnion cells with cytomegalovirus
Author(s) -
Figueroa Manuel E.,
Geder Laszlo,
Rapp Fred
Publication year - 1978
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890020410
Subject(s) - virology , syncytium , amnion , biology , human cytomegalovirus , fibroblast , virus , immunofluorescence , cytomegalovirus , cytoplasm , titer , antigen , in vitro , embryo , viral replication , cell culture , microbiology and biotechnology , herpesviridae , antibody , viral disease , immunology , pregnancy , fetus , genetics , biochemistry
Human cytomegalovirus (CMV), known to replicate in vitro in human fibroblastic cells, was found to replicate in epithelial human amnion (HA) cells. Large syncytia formed in these cells after infection with CMV; inclusion bodies were observed in the nuclei, and CMV antigens were demonstrated in both the cytoplasm and the nucleus by indirect immunofluorescence techniques. The synthesis of virus DNA was also detected, and the production of infectious virus was followed. The titers were lower (from 10 4 to 6 × 10 5 using different isolates of CMV) than those obtained in human embryo fibroblast (HEF) cells, and the replication cycle was slower in HA than in HEF cells.