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Thermal treatment and infectivity of hepatitis A virus in human feces
Author(s) -
Peterson D. A.,
Wolfe L. G.,
Larkin E. P.,
Deinhardt F. W.
Publication year - 1978
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890020303
Subject(s) - feces , hepatitis a vaccine , virology , seroconversion , hepatitis a , shellfish , infectivity , hepatitis , biology , microbiology and biotechnology , hepatitis a virus , virus , aquatic animal , fishery , fish <actinopterygii>
The susceptibility of white‐lipped marmoset monkeys (Saguinus sp) to human hepatitis A virus (HAV) provides a system for evaluation of thermal inactivation of HAV in feces and contaminated shellfish. Intramuscular or oral administration of HAV derived from feces of four patients with acute hepatitis A induced hepatitis in 28–100% of the inoculated marmosets. A 10% (w/v) fecal pool (GBG‐BM) prepared from two patients (GBG and GBM) induced hepatitis in marmosets (2/4 with 1 ml; 2/2 with 3 ml) when given orally as a 1:3 dilution. A HAV‐baby food raw oyster mixture fed to fasted marmosets induced hepatitis in 1/4 and seroconversion in 2/4 animals. Two groups of oysters were injected with HAV (concentrated 3:1 by centrifugation of the GBG‐BM pool); one group was treated at 140°F for 19 minutes and the other served as an untreated control. In animals fed the untreated inoculum, 4/6 developed hepatitis and 6/6 seroconverted, whereas of those fed the heattreated inoculum 1/7 developed hepatitis and 2/7 seroconverted. These data suggest that pasteurization methods could be developed that would eliminate shellfish‐associated hepatitis A and retain the palatability of the shellfish.