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Relationship of human adenoviruses 12, 18, and 31 as determined by hemagglutination inhibition
Author(s) -
Wigand R.,
Keller Doris
Publication year - 1978
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890020208
Subject(s) - antiserum , heterologous , virology , hemagglutination , titer , antigen , hemagglutination assay , serotype , virus , biology , neutralization , homologous chromosome , microbiology and biotechnology , immunology , gene , genetics
Adenoviruses 12 and 31, but not Ad 18, agglutinate rat blood cells at high titer, providing suitable blood cells be available and a prolonged contact period of virus with the erythrocytes is allowed. Purified virus particles show direct, and virus‐free supernatants show direct and indirect, hemagglutination, ie, enhancement of HA by heterologous antiserum. Hemagglutination inhibition with rabbit antisera shows cross‐reactions between Ad12 and Ad31 with liters 4–32 times lower than with homologous antigens; Ad18 antisera react with antigens from both of the other serotypes. No cross‐reactions were seen with antisera from other adenoviruses. This suggests an antigenic relationship of the three viruses of subgroup IV in their fiber antigen γ, in addition to the known relation in the hexon (ϵ), which is apparent in cross‐neutralization.