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Enhanced parainfluenza I (6/94) virus detection in latently infected human brain cell cultures by treatment with cytochalasin D and dimethyl sulfoxide
Author(s) -
Wroblewska Zofia,
Wellish Mary,
Gilden Donald H.
Publication year - 1978
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890020207
Subject(s) - virology , virus , biology , cell culture , cytochalasin d , dimethyl sulfoxide , vero cell , cell , viral replication , microbiology and biotechnology , cytochalasin , chemistry , biochemistry , genetics , organic chemistry , cytoskeleton
The ability of cytochalasin D (CD) and dimethyl sulfoxide (DMSO) to enhance parainfluenza I (6/94) virus replication was studied in various cell culture systems. Treatment of CV 1 cells with CD (1 μg/ml) dissolved in DMSO prior to primary 6/94 virus exposure at 10 0 –10 5 multiplicities of infection did not substantially enhance virus replication. However, there was a transient increase in cell associated virus one day after infection of DMSO‐treated cultures. CD treatment of cultures of human brain cells latently infected with 6/94 virus (LIHB cells) did not enhance 6/94 virus detection. Cocultivation of CV 1 cells with CD‐treated LIHB cell cultures, and cocultivation of LIHB cell cultures with CD‐treated CV 1 cells, resulted in the production of both cell‐associated and cell‐free 6/94 virus three and five days after cocultivation. No virus was detected after similar cocultivation of untreated LIHB cell cultures with untreated CV 1 cells. The usefulness of CD‐DMSO treatment in the rescue of virus from 6/94 LIHB cell cultures appears limited to a cocultivation system. The use of these techniques to enhance virus rescue from human tissues suspected of harboring latent viral genomes is discussed.