Premium
Cytomegalovirus proteins: II. Polypeptide composition of cytomegalovirus complement‐fixing antigen
Author(s) -
Fiala Milan,
Heiner Douglas C.,
Murnane John,
Guze Lucien B.
Publication year - 1978
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890020107
Subject(s) - antigen , cytomegalovirus , chemistry , peptide , antibody , agarose , molecular mass , virology , solubilization , epitope , microbiology and biotechnology , urea , biology , biochemistry , herpesviridae , virus , immunology , viral disease , enzyme
Complement‐fixing (CF) antigen, prepared from cytomegalovirus (CMV)‐ infected cells and solubilized by urea and Tween‐20, was fractionated on agarose gel columns. Its molecular weight was 1.5−5 × 10 7 daltons, and it contained 5 major peptides with molecular weights from 60,000–90,000 daltons. The peptide with a molecular weight of 70,000 daltons which was present in the solubilized CF antigen was absent in a soluble fraction from infected cells without CF activity and may be, therefore, important in this respect. The antigens present in the CF antigen did not elicit neutralizing antibody.