Premium
Serological relatedness of monkeypox, variola, and vaccinia viruses
Author(s) -
Esposito Joseph J.,
Obijeski John F.,
Nakano James H.
Publication year - 1977
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890010107
Subject(s) - monkeypox , virology , vaccinia , immunodiffusion , complement fixation test , antiserum , biology , antigen , poxviridae , orthopoxvirus , embryonated , heterologous , variola virus , serology , virus , ouchterlony double immunodiffusion , cowpox , microbiology and biotechnology , antibody , immunology , recombinant dna , biochemistry , gene
Closely related human and monkey orthopoxviruses were differentiated by serologic techniques. Antiviral sera were tested by immunodiffusion for reactivity against six different viral antigens prepared from either infected cell cultures or infected chorioallantoic membranes (CAMs) of embryonated eggs. Portions of each antiserum were separately absorbed with heterologous antigens from infected CAMs to remove common reactivity. The absorbed sera formed immunodiffusion precipitates with both types of antigen preparation and revealed specific‐character differences that made it possible to classify the viruses as variola, vaccinia, or monkeypox. Cross‐complement fixation tests were also used to examine the immunologic reactivities of antisera to detergent‐treated, purified preparations of three orthopoxviruses. Only common reactivities were detected by this method, however, and differentiating reactivities were not observed.