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Coupled particle light scattering: a new technique for serodiagnosis of Epstein‐Barr virus infection
Author(s) -
Baetens D.G.A.,
Van Renterghem L.M.L.
Publication year - 2001
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1080
Subject(s) - virology , serology , virus , antibody , antigen , epstein–barr virus , immunoglobulin m , multiplex , epstein–barr virus infection , immunology , immunoglobulin g , medicine , biology , bioinformatics
The Coupled Particle Light Scattering™ technique was evaluated for serological diagnosis of Epstein‐Barr Virus (EBV) infection. Two hundred ninety‐six patient sera selected from several clinical categories (acute infection, non‐primary infection, interfering non‐EBV infection, non‐infected) were tested for IgM and IgG antibodies (anti‐VCA, anti‐EBNA and anti‐EA). Determination of EBV IgG with Copalis multiplex was accurate when compared with Enzygnost ® Anti‐EBV/IgG ELISA. Although the sensitivity of Copalis IgM for acute infections was 100% a positive IgM result did not always indicate an acute infection. Strong reactivity to IgG EA (ratio 3, 1) and IgG VCA (ratio 13, 3) correlated with persistent infection or reactivation. The Copalis™I has many advantages over the existing methods, such as the possibility to measure three semi‐quantitative IgG responses to three different EBV antigens simultaneously. J. Med. Virol. 64:519–525, 2001. © 2001 Wiley‐Liss, Inc.