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Complete replication of human cytomegalovirus in explants of first trimester human placenta
Author(s) -
Gabrielli Liliana,
Losi Luisa,
Varani Stefania,
Lazzarotto Tiziana,
Eusebi Vincenzo,
Landini Maria Paola
Publication year - 2001
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1077
Subject(s) - human cytomegalovirus , virology , placenta , biology , explant culture , viral replication , cytomegalovirus , virus , betaherpesvirinae , stromal cell , andrology , fetus , herpesviridae , pregnancy , viral disease , medicine , in vitro , genetics , cancer research
Abstract Tissue integrity and viability of first trimester placenta explants were obtained in culture for 3 weeks. Explants were infected with human cytomegalovirus (HCMV), several cycles of HCMV replication were obtained and the progression of the infection was observed within a tissue that maintains its normal cellular organization. In agreement with recent clinical data, 3 weeks were necessary for the virus to colonize the placenta fully. Complete HCMV replication was observed in trophoblasts, followed by subsequent transmission of the infection to the stromal fibroblasts and fetal endothelial capillary cells. Viral DNA replication was monitored and the production of infectious viral progeny documented. J. Med. Virol. 64:499–504, 2001. © 2001 Wiley‐Liss, Inc.