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Isolation and molecular identification of echovirus 13 isolated from patients of aseptic meningitis in Korea, 2002
Author(s) -
Cheon DooSung,
Lee Jiwon,
Lee Kangbum,
Lee Sunhwa,
Park Kwisung,
Ahn Jungbae,
Jee Youngmee,
Yoon Jaedeuk,
Cho Haewol
Publication year - 2004
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.10543
Subject(s) - aseptic meningitis , echovirus , virology , isolation (microbiology) , meningitis , identification (biology) , virus isolation , picornaviridae , medicine , enterovirus , biology , microbiology and biotechnology , virus , pediatrics , botany
During 2002, several epidemics of aseptic meningitis were attributed to echovirus 13 in Korea. The causative agents of these outbreaks were isolated and identified using rhabdosarcoma cells, HEp‐2 and Buffalo green monkey kidney cells, and a neutralization test using monospecific antiserum. Fifty‐four echovirus 13 isolates were isolated from patients with aseptic meningitis in the provinces, Seoul, Kyonggi, Gwangju, Jeonju, Busan, and Ulsan. Symptoms associated with aseptic meningitis infection in patients included the occurrence of headaches and mild fever. Molecular characterization of echovirus 13 samples was achieved by sequence and phylogenetic analyses on partial VP1 sequences from 20 Korean isolates and 10 foreign isolates listed in Genbank. Minor variation was observed among the Korean isolates, which formed a unique cluster with isolates of German and Japanese origin. The marked similarities between isolates could be attributed to a relatively recent arrival of the virus in Korea. This is the first such investigation of aseptic meningitis caused by echovirus 13 on the Korean peninsula. J. Med. Virol. 73:439–442, 2004. © 2004 Wiley‐Liss, Inc.