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Hepatitis B virus genotypes and HBsAg subtypes in refugees and injection drug users in the United States determined by LiPA and monoclonal EIA
Author(s) -
Swenson Paul D.,
Van Geyt Caroline,
Alexander E. Russell,
Hagan Holly,
FreitagKoontz Jayne M.,
Wilson Shari,
Norder Heléne,
Magnius Lars O.,
Stuyver Lieven
Publication year - 2001
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1051
Subject(s) - subtyping , virology , genotype , genotyping , hbsag , monoclonal antibody , hepatitis b virus , biology , monoclonal , immunoassay , hepadnaviridae , microbiology and biotechnology , virus , antibody , immunology , genetics , gene , computer science , programming language
Hepatitis B virus (HBV) genotyping and hepatitis B surface antigen (HBsAg) subtyping were carried out on sera from 196 HBsAg‐positive patients, including 151 refugees entering the United States and 45 injection drug users in Seattle. HBsAg subtyping was performed by enzyme immunoassay (EIA) using a panel of monoclonal antibodies and the HBV genotype was determined by polymerase chain reaction (PCR) followed by detection of amplified HBV DNA by a reverse‐phase hybridization line probe assay (LiPA) using genotype‐specific probes. HBV DNA was detected by PCR in 155 (79%) of the 196 sera and all 155 were genotyped by LiPA. Samples from Southeast Asia were predominantly genotype B/subtype ayw1 and genotype C/ adr ; samples from the former Soviet Union and eastern Europe were mostly genotype D/ ayw2 and genotype D/ ayw3 ; samples from east Africa were mainly genotype A/ adw2 and genotype D/ ayw2 ; and samples from injection drug users were mostly genotype D/ ayw3 and genotype A/ adw2 . Some strains of ayw3 gave atypical monoclonal antibody reactivity patterns in the subtyping assay due to a Val/Ala instead of a Thr at amino acid residue 118 and a Thr instead of a Met at residue 125. A strain of ayw2 also gave an atypical monoclonal antibody reactivity pattern due to an Ala instead of a Thr at amino acid residue 123. LiPA genotyping and monoclonal EIA subtyping can provide useful information for epidemiological studies. J. Med. Virol. 64:305–311, 2001. © 2001 Wiley‐Liss, Inc.