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Determination of HBsAg subtypes in Western Siberian part of Russia
Author(s) -
Netesova I.G.,
Swenson P.D.,
Osipova L.P.,
Gubina M.A.,
Posukh O.L.,
Netesov S.V.
Publication year - 2003
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.10468
Subject(s) - hbsag , virology , subtyping , counterimmunoelectrophoresis , immunoassay , antibody , antigen , hepatitis b , medicine , biology , immunology , hepatitis b virus , virus , computer science , programming language
A set of monoclonal antibodies with specificity for hepatitis B surface antigen (HBsAg) was used for subtyping this antigen in sera from indigenous natives, blood donors, and drug users in Western Siberia with a modified commercial enzyme immunoassay kit for HBsAg detection. Three subtypes of HBsAg in a ratio of 36 (78%) ayw2 :8 ayw3varB (18%):2 (4%) adw2 were found in 46 (100%) HBsAg‐positive sera of different aboriginal populations of Western Siberia: the Tundra Nenets, Northern Khanty, Southern Altaians, and Kazakhs. Four subtypes of HBsAg in a ratio of 81 (57%) ayw2 :58 (15 ayw3varA and 43 ayw3varB ; 44%):2 (1%) adw2 were detected in 141 (100%) samples of blood donors from ten cities of Western Siberia. Three subtypes of HBsAg in a ratio of 34 ayw3 :(both variants, 33 ayw3varA and 1 ayw3varB ; 97.1%):1 (2.9%) ayw2 were found in blood of 35 injection drug users in Novosibirsk. J. Med. Virol. 71:183–187, 2003. © 2003 Wiley‐Liss, Inc.