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Saccharomyces cerevisiae ‐derived HBsAg preparations differ in their attachment to monocytes, immune‐suppressive potential, and T‐cell immunogenicity
Author(s) -
Vanlandschoot Peter,
Van Houtte Freya,
Hoek Frans,
Nieuwland Rienk,
LerouxRoels Geert
Publication year - 2003
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.10425
Subject(s) - immunogenicity , immune system , hbsag , virology , saccharomyces cerevisiae , biology , immunology , virus , yeast , hepatitis b virus , biochemistry
Expression of the hepatitis B virus S protein results in the formation of a lipoprotein particle, the hepatitis B surface antigen (HBsAg). Such particles, produced in Saccharomyces cerevisiae , bind to the cell surface of monocytes through interaction with the lipopolysaccharide binding protein and the lipopolysaccharide receptor, CD14. This attachment is suggested to depend on the presence of charged phospholipids in the particles. In addition, such particles interfere with the lipopolysaccharide and interleukin‐2‐induced activation of monocytes. In the present study, it is reported that of three Saccharomyces cerevisiae ‐derived HBsAg preparations, two have a reduced capacity to bind to monocytes. A correlation with a reduced potential to inhibit the lipopolysaccharide‐induced activation of monocytes and an increased potential to stimulate HBsAg‐specific T‐cell proliferation is observed. Surprisingly, differences in phospholipid content that might explain these observations, were not detected. J. Med. Virol. 70:513–519, 2003. © 2003 Wiley‐Liss, Inc.