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Interaction of human herpesvirus 6 with human CD34 positive cells
Author(s) -
Isomura Hiroki,
Yoshida Mariko,
Namba Hikaru,
Yamada Masao
Publication year - 2003
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.10415
Subject(s) - cd34 , haematopoiesis , biology , peripheral blood mononuclear cell , progenitor cell , population , thrombopoietin , microbiology and biotechnology , cord blood , stem cell , megakaryocyte , virology , in vitro , immunology , genetics , medicine , environmental health
We reported previously that human herpesvirus 6 (HHV‐6) suppresses hematopoietic colony formation of erythroid (BFU‐E), granulocyte/macrophage (CFU‐GM), and megakaryocyte (CFU‐Meg) lineages in vitro. Here we describe the interaction between HHV‐6 and human CD34+ cells, which are a major source of hematopoietic progenitor cells. CD34+ cells were immunomagnetically isolated from cord blood mononuclear cells using anti‐CD34+ antibodies coated onto either Dynabeads™ or MACS beads. The CD34+ population selected with Dynabeads showed a broad range of fluorescence. The population selected with MACS beads showed a narrow range of fluorescence. After infection with HHV‐6, two transcripts of the immediate early genes were detected with both cell populations. HHV‐6 suppressed colony formation of BFU‐E, CFU‐GM, and CFU‐Meg. HHV‐6 suppressed cell growth after 3 to 7 days culture in the presence of thrombopoietin (TPO). More differentiated CD34+ cells were more susceptible to the effects of HHV‐6. These data indicate that the targets for hematopoietic suppression by HHV‐6 are the differentiated cells. J. Med. Virol. 70:444–450, 2003. © 2003 Wiley‐Liss, Inc.