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Measurement of cell‐mediated immunity with a varicella‐zoster virus‐specific interferon‐γ ELISPOT assay: Responses in an elderly population receiving a booster immunization
Author(s) -
Smith Jeffrey G.,
Levin Myron,
Vessey Rupert,
Chan Ivan S.F.,
Hayward Anthony R.,
Liu Xu,
Kaufhold Robin M.,
Clair James,
Chalikonda Ira,
Chan Christina,
Bernard Maryann,
Wang William W.,
Keller Paul,
Caulfield Michael J.
Publication year - 2003
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.10318
Subject(s) - elispot , medicine , immunology , virology , varicella zoster virus , booster dose , vaccination , immune system , population , immunization , peripheral blood mononuclear cell , immunity , booster (rocketry) , chickenpox vaccine , varicella vaccine , virus , biology , t cell , biochemistry , environmental health , in vitro , physics , astronomy
An interferon‐gamma ELISPOT assay has been developed for assessment of cellular immune responses to Varicella‐Zoster Virus (VZV) in large, multi‐center clinical vaccine trials. We show that the assay performed best when testing peripheral blood mononuclear cells (PBMCs) that had been isolated and then frozen on the same day as blood was drawn, and that freezing PBMCs from blood that was stored overnight before processing resulted in dramatically reduced responses. This assay was used to monitor cell‐mediated immunity (CMI) in response to a booster immunization with an investigational live, attenuated VZV vaccine in an elderly population that had been vaccinated 8–10 years previously. The booster vaccine elicited a 1.6‐ to 1.7‐fold rise in the VZV‐specific cellular immune response as measured by the ELISPOT assay. The increase from pre to post booster vaccination response was more pronounced (∼ 2.2‐fold rise) in a subset of subjects who had received two prior immunizations with a live, attenuated vaccine. J. Med. Virol. 70:S38–S41, 2003. © 2003 Wiley‐Liss, Inc.