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Induction of cellular immunity to varicella‐zoster virus glycoproteins tested with pernasal coadministration of escherichia coli enterotoxin in mice
Author(s) -
Tsuji Takao,
Shiraki Kimiyasu,
Sato Hitoshi,
Sasaki Keiko,
Arita Michiko,
Kato Michio,
Takahashi Tsuyoshi,
Ochi Sadayuki,
Ichinose Yoshio,
Yokochi Takashi,
Asano Yoshizo
Publication year - 2003
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.10309
Subject(s) - heat labile enterotoxin , enterotoxin , cellular immunity , virology , microbiology and biotechnology , spleen , biology , immunity , adjuvant , immunization , virus , glycoprotein , escherichia coli , immune system , immunology , biochemistry , gene
A mutant of Escherichia coli enterotoxin promotes the induction of cellular immunity to a live varicella vaccine (the Oka strain) as a mucosal adjuvant in mice. An investigation was carried out to determine which of the purified glycoproteins of the virus among three induced cellular immunity with a single nasal administration. Spleen cells from mice immunized nasally with the vaccine and toxin produced interleukin‐2 (IL‐2) at the same level on restimulation in vitro with glycoprotein H: glycoprotein L (gH:gL), gB, and gE:gI, but not IL‐4. The spleen cells from mice immunized with gH:gL, gB, or gE:gI and toxin produced IL‐2 on restimulation with gH:gL, gB, or gE:gI, respectively, and the vaccine, but not IL‐4. Immunization with gH:gL and the toxin showed increased thymidine uptake and production of IL‐2 and interferon‐γ (IFN‐γ) of the spleen cells, but not IL‐4, depending on the dose of gH:gL used for immunization and restimulation in vitro. Purified gE:gI and gB have been reported to be the strongest stimulators of cellular immunity to varicella upon subcutaneous injection and are useful as a subunit vaccine. All the glycoproteins tested are excellent stimulators of cellular immunity to the virus and itself on nasal co‐immunization with the toxin. J. Med. Virol. 69:451–458, 2003. © 2003 Wiley‐Liss, Inc.