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Human antibody response to Toscana virus glycoproteins expressed by recombinant baculovirus
Author(s) -
Di Bonito Paola,
Bosco Simona,
Mochi Stefania,
Accardi Luisa,
Ciufolini Maria G.,
Nicoletti Loredana,
Giorgi Colomba
Publication year - 2002
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.10227
Subject(s) - virology , glycoprotein , recombinant dna , virus , western blot , recombinant virus , biology , nucleoprotein , antibody , baculoviridae , immune system , humoral immunity , antigen , viral envelope , microbiology and biotechnology , immunology , gene , spodoptera , biochemistry
The arthropod‐borne Toscana virus has been associated with acute neurological disease in humans. In this study, the viral envelope glycoproteins were expressed in soluble form in a baculovirus system. The recombinant sGN and sGC proteins were used as viral antigens in a Western blot assay to analyze the specific immune response in sera from patients with recognized virus‐associated aseptic meningitis. The anti‐glycoprotein and the anti‐nucleoprotein N IgG responses were compared by an immunoassay based on the recombinant proteins. In this system, all the sera showed a high reactivity to the N protein, but they differed in the response to the glycoproteins. J. Med. Virol. 68:615–619, 2002. © 2002 Wiley‐Liss, Inc.