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Genotypes and S‐gene variability of Mexican hepatitis B virus strains
Author(s) -
Sánchez L.V.,
Maldonado M.,
BastidasRamírez B.E.,
Norder H.,
Panduro A.
Publication year - 2002
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.10166
Subject(s) - virology , genotype , biology , gene , virus , hepatitis b virus , hepadnaviridae , hepatitis a virus , genetics , orthohepadnavirus , hepatitis c virus
The genotypes and subtypes of 15 Mexican hepatitis B virus strains were determined by sequencing and phylogenetic analysis of the small S‐gene. The most predominant strains were found to be divergent genotype/subtype F/ adw4 strains (66.6%), followed by A/ adw2 (20.0%), D/ ayw3 (6.7%), and G/ adw2 (6.7%). The S‐genes of the Mexican genotype F strains and two Nicaraguan strains described previously formed a subcluster with more than 4% divergence from the other strains within this genotype. The Mexican strains within genotypes A and D showed the highest homology with strains from Europe and the United States. Ten amino acid substitutions not described previously were found in the S‐genes of strains from nine chronic carriers, whereas the S gene in strains from six acute hepatitis B patients were highly conserved as compared to their respective genotypes. One genotype F strain from an HBsAg positive chronic carrier had a T to A mutation at position 647, forming a translational stop at codon 216. Two genotype F strains from HBsAg negative chronic carriers had a Val180 instead of an Ala found in the other genotype F strains. This study shows that a divergent genotype F predominates in Mexican strains analyzed, which presented amino acid substitutions not reported previously outside the a determinant. J. Med. Virol. 68:24–32, 2002. © 2002 Wiley‐Liss, Inc.