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Influence of cation adduction on the separation characteristics of flavonoid diglycoside isomers using dual gate‐ion mobility‐quadrupole ion trap mass spectrometry
Author(s) -
Clowers Brian H.,
Hill Herbert H.
Publication year - 2006
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.994
Subject(s) - chemistry , hesperetin , quadrupole ion trap , aglycone , hesperidin , ion trap , flavonoid , mass spectrometry , electrospray ionization , chromatography , rutin , stereochemistry , organic chemistry , glycoside , medicine , alternative medicine , pathology , antioxidant
An electrospray ionization‐dual gate‐ion mobility‐quadrupole ion trap mass spectrometer was used to evaluate the separation characteristics of isomeric flavonoid diglycosides adducted with sodium, potassium, and silver. This instrumental configuration allows ions to be selectively accumulated within the ion trap on the basis of their gas phase conformation prior to mass analysis. For the metal cations examined, silver produced the most compact adducts with flavonoid diglycosides. Listed in order of increasing size, the trend of flavonoid diglycoside ion‐neutral cross sections adducted with Na + , K + , and Ag + was narirutin < naringin < hesperidin < neohesperidin < rutin. To examine the separation contribution of the carbohydrate group, hesperetin, the aglycone of hesperidin, and neohesperin were compared to quercetin, the aglycone of rutin. Separation of the flavonoid diglycosides indicated that quercetin‐derived diglycosides drifted longer than their hesperetin‐derived isomers. Combined with the observed collision assisted dissociation (CAD) data, these findings suggest that carbohydrate moiety plays a significant role in both the separation and metal chelating characteristics of flavonoid diglycosides. Copyright © 2006 John Wiley & Sons, Ltd.