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Differentiation of diiodothyronines using electrospray ionization tandem mass spectrometry
Author(s) -
Zhang Yuntao,
Conrad Abigail H.,
Thoma Rudy,
Conrad Gary W.
Publication year - 2006
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.971
Subject(s) - chemistry , electrospray ionization , ionization , fragmentation (computing) , tandem mass spectrometry , mass spectrometry , chemical ionization , chromatography , analytical chemistry (journal) , ion , organic chemistry , computer science , operating system
Abstract Diiodothyronines 3,5‐diiodothyronine (3,5‐T2), 3′,5′‐diiodothyronine (3′,5′‐T2), and 3,3′‐diiodothyronine (3,3′‐T2) are important metabolites of 3,5,3′‐triiodothyronine (T3) and 3,3′,5′‐triiodothyronine (rT3; reverse T3). In this paper, a novel and rapid method for identifying and quantifying 3,5‐T2, 3′,5′‐T2 and 3,3′‐T2 has been introduced using electrospray ionization tandem mass spectrometry (ESI‐MS/MS). Fragmentation patterns were proposed on the basis of our data obtained by ESI‐MS/MS. MS 2 spectra in either negative ionization mode or positive ionization mode can be used to differentiate 3,5‐T2, 3′,5′‐T2 and 3,3′‐T2. On the basis of the relative abundance of fragment ions in MS 2 spectra under the positive ionization mode, quantification of the 3,5‐T2, 3′,5′‐T2 and 3,3′‐T2 isomers in mixtures is also achieved without prior separation. Copyright © 2005 John Wiley & Sons, Ltd.