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Location and base selectivity on fragmentation of brominated oligodeoxynucleotides
Author(s) -
Buré Corinne,
Castaing Bertrand,
Lange Catherine,
Delmas Agnès F.
Publication year - 2006
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.967
Subject(s) - chemistry , fragmentation (computing) , bromine , base (topology) , selectivity , stereochemistry , organic chemistry , mathematical analysis , mathematics , computer science , operating system , catalysis
Bromine‐modified oligodeoxynucleotides (ODNs) were fragmented in the electrospray source to study the influence of brominated bases on fragmentation. Several 13‐mer ODNs containing a brominated pyrimidine base, BrdU (5‐bromodeoxyuridine) or BrdC (5‐bromodeoxycytidine), were analyzed. Low cone voltage fragmentation yields a loss of the brominated base with a preferential loss for the brominated base closer to the 5′‐end (2‐position > 4‐position > 12‐position) as well as a preferential loss of BrdU over BrdC. Higher cone voltage produces backbone fragmentation with complementary a n ‐base and w m ions close to the brominated base. On the basis of these observations, we located the brominated base in the sequence for all of the ODNs studied. Copyright © 2005 John Wiley & Sons, Ltd.