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TRH‐like antidepressant peptide, pyroglutamyltyroslyprolineamide, occurs in rat brain
Author(s) -
Eugene Pekary A.,
Faull Kym F.,
Paulson Mark,
Lloyd Robert L.,
Sattin Albert
Publication year - 2005
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.904
Subject(s) - chemistry , peptide , radioimmunoassay , anticonvulsant , high performance liquid chromatography , in vivo , medicine , endocrinology , chromatography , biochemistry , epilepsy , psychology , microbiology and biotechnology , neuroscience , biology
We have previously reported the occurrence of pGlu‐Glu‐Pro‐NH 2 (Glu‐TRH, EEP), Val‐TRH, Tyr‐TRH, Leu‐TRH, Phe‐TRH, and Trp‐TRH in rat brain using a combination of HPLC and radioimmunoassays with antibodies that cross‐react with the general structure pGlu‐X‐Pro‐NH 2 where ‘X’ maybe any amino acid residue ( Peptides 2004; 25 : 647). This new family of TRH‐like peptides, along with TRH (pGlu‐His‐Pro‐NH 2 ), has neuroprotective, anticonvulsant, antidepressant, euphoric, anti‐amnesic, and analeptic effects. We now report that a combination of affinity chromatography using a rabbit antibody specific for Tyr‐TRH and Phe‐TRH, along with HPLC and tandem mass spectrometry operating in the multiple reaction monitoring (MRM) mode, provide conclusive evidence for the presence of Tyr‐TRH in rat brain. Furthermore, synthetic Tyr‐TRH is active in the Porsolt Swim Test suggesting that it is a fourth member of this family of in vivo neuroregulatory agents that have psychopharmacotherapeutic properties. Copyright © 2005 John Wiley & Sons, Ltd.