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MALDI‐TOF mass spectrometric analysis for the characterization of the 5,10,15,20‐tetrakis‐ ( m ‐hydroxyphenyl)bacteriochlorin ( m ‐THPBC) photoproducts in biological environment
Author(s) -
Lassalle HenriPierre,
Lourette Natacha,
Maunit Benoît,
Muller JeanFrançois,
Guillemin François,
BezdetnayaBolotine Lina
Publication year - 2005
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.886
Subject(s) - chemistry , photobleaching , photosensitizer , mass spectrometry , absorption (acoustics) , synthon , absorption spectroscopy , nuclear chemistry , spectroscopy , ultraviolet visible spectroscopy , chromatography , photochemistry , stereochemistry , organic chemistry , fluorescence , physics , quantum mechanics , acoustics
Abstract Photoproducts formation upon irradiation (739 nm) of 5,10,15,20‐tetrakis( m ‐hydroxyphenyl)bacteriochlorin ( m ‐THPBC) in phosphate buffer saline (PBS) supplemented with human serum albumin (HSA) were studied by means of absorption spectroscopy and MALDI‐TOF mass spectrometry. The experiments were performed with a freshly prepared PBS–HSA solution of m ‐THPBC and with a PBS–HSA m ‐THPBC solution incubated for 6 h at 37 °C. The incubation of m ‐THPBC solution leads to the dye monomerisation, whereas in the freshly prepared solution, m ‐THPBC is under an aggregated form. Regardless of the incubation condition, photobleaching experiments carried out by absorption spectroscopy demonstrate the degradation of the photosensitizer and its phototransformation in m ‐THPC. Moreover, m ‐THPC was the sole photoproduct detected using absorption spectroscopy. Together with a degradation of m ‐THPBC and formation of m ‐THPC, MALDI‐TOF mass spectrometry evidenced several other photoinduced modifications. Photoproducts such as dihydroxy m ‐THPBC and dihydroxy m ‐THPC were detected in both conditions; however, the formation of hydroxylated photoproducts was significantly greater in incubated solution. In addition, small molecules arising from the degradation of the photosensitizer and identified as dipyrin derivatives and dipyrrolic synthon were observed. Copyright © 2005 John Wiley & Sons, Ltd.