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Determination of zinc to beta‐peptide binding constants with electrospray ionization mass spectrometry
Author(s) -
Wortmann Arno,
Rossi Francesco,
Lelais Gerald,
Zenobi Renato
Publication year - 2005
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.852
Subject(s) - chemistry , electrospray ionization , mass spectrometry , zinc , titration , electrospray , chromatography , binding constant , analytical chemistry (journal) , stability constants of complexes , peptide , ionization , inorganic chemistry , binding site , organic chemistry , ion , aqueous solution , biochemistry
Abstract We present an improvement of the titration method for binding constant determination with electrospray ionization (ESI) mass spectrometry that is unaffected by differences in ESI response of measured species in solution. The method consists of a calibration and titration, both using an internal standard that allows relative quantitation. This avoids artifacts such as a decrease in overall signal intensity with increasing ligand concentrations, rendering this approach more reliable and meaningful than direct evaluation of ESI peak intensities. We demonstrate the de novo binding constant determination of novel zinc binding beta‐peptides, which have been synthesized with the goal of creating secondary structures stabilized by metal complexation. Copyright © 2005 John Wiley & Sons, Ltd.