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Mass spectrometry in the study of anthocyanins and their derivatives: differentiation of Vitis vinifera and hybrid grapes by liquid chromatography/electrospray ionization mass spectrometry and tandem mass spectrometry
Author(s) -
Mazzuca Pierluca,
Ferranti Pasquale,
Picariello Gianluca,
Chianese Lina,
Addeo Francesco
Publication year - 2005
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.778
Subject(s) - anthocyanin , chemistry , anthocyanidin , vitis vinifera , mass spectrometry , cyanidin , chromatography , tandem mass spectrometry , berry , cultivar , liquid chromatography–mass spectrometry , pigment , food science , botany , organic chemistry , biology
A mass spectrometric‐based procedure for anthocyanin profiling was set up to distinguish authentic Vitis vinifera from hybrid red grapevine cultivars. 3‐ O ‐Monoglucoside and the related acetyl‐, p ‐coumaryl‐ and caffeoyl‐monoglucoside anthocyanins occurred only in Vitis vinifera , whereas 3,5‐ O ‐diglucoside and the substituted acetyl‐, p ‐coumaryl‐, feruloyl‐ and caffeoyl‐diglucoside anthocyanins were the additional pigments in hybrid grapevines. The procedure was applied expressly to identify red grape cultivars based on the anthocyanin chemo‐type determination. In particular, a red grape cultivar, having 3,5‐ O ‐diglucoside anthocyanins and a novel class of anthocyanin monoglucosides, such as cyanidin‐3‐ O ‐, cyanidin‐3‐ O ‐(6‐ O ‐acetyl)‐ and cyanidin‐3‐ O ‐(6‐ O ‐ p ‐coumaryl)pentoside, was classified as hybrid. A second vine cultivar, characterized exclusively by 3‐ O ‐monoglucoside anthocyanins, was included among the Vitis vinifera species. Anthocyanin profiling by mass spectrometry could represent the core of a chemotaxonomic procedure for distinguishing American and European grapevines based on the identification of post‐synthetic anthocyanidin modification. Copyright © 2004 John Wiley & Sons, Ltd.