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Monitoring of immune response by blood serum profiling using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Haag Anthony M.,
Chaiban Joseph,
Johnston Kenneth H.,
Cole Richard B.
Publication year - 2001
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.76
Subject(s) - chemistry , mass spectrometry , immune system , enterococcus faecalis , chromatography , matrix assisted laser desorption/ionization , desorption , whole blood , matrix (chemical analysis) , analytical chemistry (journal) , biochemistry , immunology , escherichia coli , organic chemistry , adsorption , gene , biology
The application of matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) to the analysis of blood serum leads to the observation of a large variety of reproducible mass spectral peaks corresponding to blood components. In this study, the use of MALDI‐TOFMS was developed as a tool for monitoring immune response to bacterial infection. Employing the MALDI‐TOFMS approach, the levels of many components of blood were found to be immune response independent whereas others were found to correlate directly with the response of the immune system to two known types of bacteria ( Staphylococcus aureus and Enterococcus faecalis ). The methodologies reported here should be useful for the rapid monitoring of blood, especially that of the immune response mechanisms in various animal species. Copyright © 2001 John Wiley & Sons, Ltd.