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Subcellular imaging mass spectrometry of brain tissue
Author(s) -
McDonnell Liam A.,
Piersma Sander R.,
Altelaar A. F. Maarten,
Mize Todd H.,
Luxembourg Stefan L.,
Verhaert Peter D. E. M.,
van Minnen Jan,
Heeren Ron M. A.
Publication year - 2005
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.735
Subject(s) - mass spectrometry imaging , chemistry , maldi imaging , mass spectrometry , analytical chemistry (journal) , analyte , secondary ion mass spectrometry , resolution (logic) , ion , polyatomic ion , matrix (chemical analysis) , matrix assisted laser desorption/ionization , desorption , chromatography , organic chemistry , adsorption , artificial intelligence , computer science
Imaging mass spectrometry provides both chemical information and the spatial distribution of each analyte detected. Here it is demonstrated how imaging mass spectrometry of tissue at subcellular resolution can be achieved by combining the high spatial resolution of secondary ion mass spectrometry (SIMS) with the sample preparation protocols of matrix‐assisted laser desorption/ionization (MALDI). Despite mechanistic differences and sampling 10 5 times less material, matrix‐enhanced (ME)‐SIMS of tissue samples yields similar results to MALDI (up to m / z 2500), in agreement with previous studies on standard compounds. In this regard ME‐SIMS represents an attractive alternative to polyatomic primary ions for increasing the molecular ion yield. ME‐SIMS of whole organs and thin sections of the cerebral ganglia of Lymnaea stagnalis demonstrate the advantages of ME‐SIMS for chemical imaging mass spectrometry. Subcellular distributions of cellular analytes are clearly obtained, and the matrix provides an in situ height map of the tissue, allowing the user to identify rapidly regions prone to topographical artifacts and to deconvolute topographical losses in mass resolution and signal‐to‐noise ratio. Copyright © 2005 John Wiley & Sons, Ltd.