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On‐line size‐exclusion chromatography/mass spectrometry of low molecular mass heparin
Author(s) -
Henriksen Jens,
Ringborg Lene Hoffmeyer,
Roepstorrf Peter
Publication year - 2004
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.723
Subject(s) - chemistry , chromatography , electrospray ionization , mass spectrometry , electrospray , heparin , size exclusion chromatography , molecular mass , analytical chemistry (journal) , organic chemistry , biochemistry , enzyme
Heparin and low molecular mass heparin (LMMH) consists of complex mixtures of sulphated linear oligosaccharides that are difficult to analyse. An on‐line size exclusion chromatographic/electrospray ionization (ESI) mass spectrometric method that allows the determination of more than 60 components in an LMMH preparation is presented. The experimental setup includes on‐line cation exchange in order to prevent massive adducting in the ESI interface. Copyright © 2004 John Wiley & Sons, Ltd.