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Simultaneous determination of capecitabine and its metabolite 5‐fluorouracil by column switching and liquid chromatographic/tandem mass spectrometry
Author(s) -
Siethoff Christoph,
Orth Matthias,
Ortling Andrea,
Brendel Erich,
WagnerRedeker Winfried
Publication year - 2004
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.655
Subject(s) - chemistry , chromatography , capecitabine , metabolite , tandem mass spectrometry , mass spectrometry , liquid chromatography–mass spectrometry , tandem , cancer , medicine , biochemistry , materials science , composite material , colorectal cancer
A liquid chromatographic/tandem mass spectrometric method was developed and validated for the quantitation of capecitabine and its metabolite 5‐fluorouracil in human plasma. The simultaneous determination of both analytes was achieved by a column switching method using a trapping column and two analytical columns with different stationary phases. Isocratic elution was used for the separation of capecitabine on a C 18 column whereas 5‐fluorouracil was separated using gradient elution on an non‐polar carbon phase. The calibration curves were linear for both compounds with a correlation factor ( R 2 ) > 0.9993 for 5‐fluorouracil and >0.9942 for capecitabine. The assay was validated in the concentration range 5.00–1000 ng ml −1 for both compounds. The intra‐day precision was better than 10% for 5‐fluorouracil and better than 11% for capecitabine whereas the inter‐day precision was better than 8% for 5‐fluorouracil and better than 14% for capecitabine. Copyright © 2004 John Wiley & Sons, Ltd.