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Oligosaccharide sequences in Quillaja saponins by electrospray ionization ion trap multiple‐stage mass spectrometry
Author(s) -
Broberg Susanna,
Nord Lars I.,
Kenne Lennart
Publication year - 2004
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.642
Subject(s) - chemistry , oligosaccharide , mass spectrometry , electrospray ionization , chromatography , ion trap , saponin , electrospray , analytical chemistry (journal) , organic chemistry , medicine , alternative medicine , pathology
Abstract Ten different samples with 13 previously identified saponin structures from Quillaja saponaria Molina were investigated by electrospray ionization ion trap multiple‐stage mass spectrometry (ESI‐ITMS n ) in positive and negative ion modes. Both positive and negative ion mode MS 1 –MS 4 spectra were analyzed, showing that structural information on the two oligosaccharide parts in the saponin can be obtained from positive ion mode spectra whereas negative ion mode spectra mainly gave information on one of the oligosaccharide parts. Analysis of MS 1 –MS 4 spectra identified useful key fragment ions important for the structural elucidation of Quillaja saponins. A flowchart involving a stepwise procedure based on key fragments from MS 1 –MS 3 spectra was constructed for the identification of structural elements in the saponin. Peak intensity ratios in MS 3 spectra were found to be correlated with structural features of the investigated saponins and are therefore of value for the identification of terminal monosaccharide residues. Copyright © 2004 John Wiley & Sons, Ltd.