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Screening for dihydropyridine calcium channel blockers in plasma by automated solid‐phase extraction and liquid chromatography/tandem mass spectrometry
Author(s) -
Mueller Claudia A.,
González Ana B. Baranda,
Weinmann Wolfgang
Publication year - 2004
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.630
Subject(s) - chemistry , chromatography , mass spectrometry , solid phase extraction , tandem mass spectrometry , selected reaction monitoring , extraction (chemistry) , liquid chromatography–mass spectrometry , triple quadrupole mass spectrometer , elution , tandem , analyte , dihydropyridine , analytical chemistry (journal) , calcium , materials science , organic chemistry , composite material
A liquid chromatographic/tandem mass spectrometric (LC/MS/MS) screening method was developed for the screening of 11 calcium channel blockers of the 1,4‐dihydropyridine type in plasma samples for forensic and clinical cases. Plasma samples were extracted by automated solid‐phase extraction. Analysis was performed using a reversed‐phase C 18 column, gradient elution and a triple‐quadrupole mass spectrometer with TurboIonSpray source in positive mode and multiple reaction monitoring. This method was found to be selective and sensitive for the detection of the target compounds at their therapeutic plasma concentrations. Copyright © 2004 John Wiley & Sons, Ltd.