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Ion‐pair assisted recovery of matrix‐assisted laser desorption/ionization mass spectral signals from SDS‐containing peptide–protein mixtures
Author(s) -
Rajnarayanan Rajendram V.,
Wang Kuan
Publication year - 2004
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.572
Subject(s) - chemistry , sodium dodecyl sulfate , mass spectrometry , matrix assisted laser desorption/ionization , desorption , chromatography , ion , analytical chemistry (journal) , matrix (chemical analysis) , mass spectrum , ionization , adsorption , organic chemistry
We have developed a simple and effective means of using alkylammonium ion‐pairing agents, such as cetyltetramethylammonium bromide, to recover matrix assisted laser desorption/ionization mass spectrometric (MALDI‐MS) signals from sodium dodecyl sulfate (SDS)‐containing protein and peptide samples. A two‐layer method of matrix preparation, with a bottom matrix layer of ion‐pairing agents and a top matrix layer of SDS protein samples, is essential for reproducible MALDI mass spectra with good recovery. Both buffer ions and ion‐pairing agents have profound effects on signal recovery and can be rapidly and systematically optimized. This practical technique, termed ion‐pair assisted recovery (IPAR), is compatible with major SDS‐based biotechniques and can be easily incorporated into high‐throughput proteomic analysis. Copyright © 2004 John Wiley & Sons, Ltd.