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Identification of anthraquinone coloring matters in natural red dyes by electrospray mass spectrometry coupled to capillary electrophoresis
Author(s) -
Puchalska Maria,
Orlińska Magdalena,
Ackacha Mohamed A.,
PołećPawlak Kasia,
Jarosz Maciej
Publication year - 2003
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.549
Subject(s) - chemistry , capillary electrophoresis , anthraquinone , chromatography , alizarin , electrospray , mass spectrometry , natural dye , detection limit , dyeing , nuclear chemistry , organic chemistry
Capillary electrophoresis with UV/visible diode‐array detection (DAD) and electrospray mass spectrometric (ESI‐MS) detection were used for the identification of anthraquinone color components of cochineal, lac‐dye and madder, natural red dyestuffs often used by ancient painters. For the purpose of such analysis, ESI‐MS was found to be a much more appropriate detection technique than DAD one owing to its higher sensitivity (detection limits in the range 0.1–0.5 µg ml −1 ) and selectivity. The method developed made it possible to identify unequivocally carminic acid and laccaic acids A, B and E as coloring matters in the examined preparations of cochineal and lac‐dye, respectively. In madder, European Rubia tinctorum , alizarin and purpurin were found. The method allows the rapid, direct and straightforward identification and quantification of components of natural products used in art and could be very helpful in restoration and conservation procedures. Copyright © 2003 John Wiley & Sons, Ltd.