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Gas‐phase investigation of Pd(II)‐alanine complexes with small native and derivatized peptides containing histidine
Author(s) -
Anbalagan V.,
Van Stipdonk M. J.
Publication year - 2003
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.512
Subject(s) - chemistry , histidine , deprotonation , electrospray ionization , peptide bond , amide , stereochemistry , peptide , imidazole , alanine , ternary complex , dissociation (chemistry) , mass spectrometry , tandem mass spectrometry , amino acid , organic chemistry , ion , chromatography , biochemistry , enzyme
We report here the generation of gas‐phase complexes containing Pd(II), a ligand (deprotonated alanine, A − ), and/or N‐terminus derivatized peptides containing histidine as one of the amino acids. The species were produced by electrospray ionization, and their gas‐phase reactions were investigated using ion‐trap tandem mass spectrometry. Pd(II) forms a stable diaqua complex in the gas phase of the formula, [Pd(A − ) (H 2 O) 2 ] + , (where A − = deprotonated alanine) along with ternary complexes containing A − and peptide. The collision‐induced dissociation (CID) patterns of the binary and ternary complexes were investigated, and the dissociation patterns for the ternary complexes suggest that: (a) the imidazole ring of the histidine side group may be the intrinsic binding site of the metal ion, and (b) the peptides fragment primarily by cleavage of the amide bond to the C‐terminal side of the histidine residues. These observations are in accord with previous solution‐state studies in which Pd(II) was shown to cause hydrolysis of an amide bond of a peptide at the same position. Copyright © 2003 John Wiley & Sons, Ltd.