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Characterization of aconitine‐type alkaloids in the flowers of Aconitum kusnezoffii by electrospray ionization tandem mass spectrometry
Author(s) -
Wang Yong,
Song Fengrui,
Xu Qingxuan,
Liu Zhiqiang,
Liu Shuying
Publication year - 2003
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.510
Subject(s) - chemistry , aconitine , aconitum , electrospray ionization , tandem mass spectrometry , mass spectrometry , fragmentation (computing) , dart ion source , collision induced dissociation , acetic acid , electrospray , chromatography , stereochemistry , alkaloid , organic chemistry , electron ionization , ionization , ion , computer science , operating system
The fragmentation mechanism of aconitine‐type alkaloids in the flowers of Aconitum kusnezoffii (FAK) was investigated using electrospray ionization tandem mass spectrometry (ESI‐MS n ) firstly. The analysis of the collision‐induced dissociation (CID) spectra of three purified aconitine standards and six previously reported aconitines indicated that the fragmentation of the protonated aconitines at low‐energy CID follows a similar pathway. The elimination of a C 8 ‐substituent such as an acetic acid or a fatty acid is the dominant fragmentation mode in MS2. Successive losses of CH 3 COOH, CH 3 OH, H 2 O, BzOH, and CO are the main fragmentation pathways of aconitine‐type alkaloids in MS 3 spectra. Based on these features, a rapid method for the direct detection and characterization of alkaloids from an ethanolic extract of FAK is described. All the known aconitum alkaloids are detected and a series of lipo‐aconitines has been found for the first time in this plant. Copyright © 2003 John Wiley & Sons, Ltd.