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Electron ionization mass spectrometric study of monomeric models of O ‐polysaccharides of Vibrio cholerae O:1, serotypes Ogawa and Inaba
Author(s) -
Kováčik Vladimír,
Pätoprstý Vladimír,
Oksman Pentti,
Mistrík Robert,
Kováč Pavol
Publication year - 2003
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.506
Subject(s) - chemistry , oxonium ion , protonation , electron ionization , mass spectrometry , fragmentation (computing) , ion , crystallography , analytical chemistry (journal) , stereochemistry , ionization , organic chemistry , chromatography , computer science , operating system
Fragmentation mechanisms of electron ionization (EI) mass spectrometry of the title compounds have been elucidated by high‐resolution (HR) mass spectrometric measurements of the elemental composition and measurements of the metastable transitions (B 2 /E, CID). The experimental results were interpreted with the help of Mass Frontier 3.0 software, which aided the elucidation of fragmentation mechanisms and helped to deduce structures of the ions formed. Characteristic under the conditions of EI‐MS measurement was the production of protonated adducts. Three distinct pathways observed include the formation of oxonium type ions, the conjugated transfer of electrons in the pyranose ring, and cleavage of the acylamide side chains. By applying the results obtained, the molecular mass, as well as the structures of both the saccharide and acylamide side chain involved in related substances, can be determined. Copyright © 2003 John Wiley & Sons, Ltd.