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Direct tissue analysis using matrix‐assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation
Author(s) -
Schwartz Sarah A.,
Reyzer Michelle L.,
Caprioli Richard M.
Publication year - 2003
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.505
Subject(s) - chemistry , mass spectrometry , matrix (chemical analysis) , sample preparation , desorption , matrix assisted laser desorption/ionization , sample (material) , analytical chemistry (journal) , surface enhanced laser desorption/ionization , mass spectrometry imaging , ionization , desorption electrospray ionization , chromatography , sample preparation in mass spectrometry , chemical ionization , electrospray ionization , ion , organic chemistry , adsorption
Practical guidelines for the preparation of tissue sections for direct analysis by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including tissue storage, sectioning and mounting are described. Emphasis is placed on optimizing matrix parameters such as the type of matrix molecule used, matrix concentration, and solvent composition. Several different techniques for matrix application are illustrated. Optimal instrument parameters and the necessity for advanced data analysis approaches with regards to direct tissue analysis are also discussed. Copyright © 2003 John Wiley & Sons, Ltd.