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Two‐laser infrared and ultraviolet matrix‐assisted laser desorption/ionization
Author(s) -
Little Mark W.,
Kim JaeKuk,
Murray Kermit K.
Publication year - 2003
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.494
Subject(s) - chemistry , matrix assisted laser desorption electrospray ionization , laser , soft laser desorption , mass spectrometry , ionization , ultraviolet , matrix assisted laser desorption/ionization , desorption , ion , analytical chemistry (journal) , yield (engineering) , ambient ionization , far infrared laser , nitrogen laser , atmospheric pressure laser ionization , matrix (chemical analysis) , infrared , ion source , ultrafast laser spectroscopy , optoelectronics , optics , photoionization , electron ionization , materials science , chromatography , physics , organic chemistry , adsorption , metallurgy
Matrix‐assisted laser desorption/ionization (MALDI) was performed using two pulsed lasers with wavelengths in the IR and UV regions. A 10.6 µm pulsed CO 2 laser was used to irradiate a MALDI target, followed after an adjustable delay by a 337 nm pulsed nitrogen laser. The sample consisted of a 2,5‐dihydroxybenzoic acid matrix and bovine insulin guest molecule. The pulse energy for both of the lasers was adjusted so that the ion of interest, either the matrix or guest ion, was not produced by either of the lasers alone. The delay time for maximum ion yield occurs at 1 µs for matrix and guest ions and the signal decayed to zero in ∼400 µs. A mechanism is presented for enhanced UV MALDI ion yield following the IR laser pulse based on transient heating. Copyright © 2003 John Wiley & Sons, Ltd.