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‘Mass defect’ tags for biomolecular mass spectrometry
Author(s) -
Hall Michael P.,
Ashrafi Siamak,
Obegi Imad,
Petesch Robert,
Peterson Jeffrey N.,
Schneider Luke V.
Publication year - 2003
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.493
Subject(s) - chemistry , mass spectrometry , biomolecule , tandem mass tag , resolution (logic) , isobaric labeling , proteomics , tandem mass spectrometry , high resolution , protein mass spectrometry , computational biology , analytical chemistry (journal) , nanotechnology , chromatography , quantitative proteomics , biochemistry , computer science , materials science , artificial intelligence , biology , gene , remote sensing , geology
We present a new class of ‘mass defect’ tags with utility in biomolecular mass spectrometry. These tags, incorporating element(s) with atomic numbers between 17 (Cl) and 77 (Ir), have a substantially different nuclear binding energy (mass defect) from the elements common to biomolecules. This mass defect yields a readily resolvable mass difference between tagged and untagged species in high‐resolution mass spectrometers. We present the use of a subset of these tags in a new protein sequencing application. This sequencing technique has advantages over existing mass spectral protein identification methodologies: intact proteins are quickly sequenced and unambiguously identified using only an inexpensive, robust mass spectrometer. We discuss the potential broader utility of these tags for the sequencing of other biomolecules, differential display applications and combinatorial methods. Copyright © 2003 John Wiley & Sons, Ltd.