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Assignment of acetyl groups to O‐2 and/or O‐3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry
Author(s) -
Quéméner Bernard,
Cabrera Pino Juan Carlos,
Ralet MarieChristine,
Bonnin Estelle,
Thibault JeanFrançois
Publication year - 2003
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.478
Subject(s) - chemistry , mass spectrometry , electrospray ionization , glycosidic bond , dissociation (chemistry) , ion , electrospray , ion trap , tandem mass spectrometry , hydrolysis , chromatography , enzyme , organic chemistry
Partially acetylated and methylated oligogalacturonides produced by enzymatic hydrolysis of sugar beet pectin were analysed by negative electrospray ionization ion trap mass spectrometry (ESI‐ITMS). The 18 O labelling of the oligomer reducing end allowed the precise assignment of the fragments resulting from glycosidic bond and cross‐ring cleavages. The collisional‐induced dissociation of the C i and Z j fragment ions through sequential MS n experiments always displayed 0, 2 A‐type cross‐ring cleavage ions which were related to C 2 H 4 O 2 losses. These 0, 2 A ions appeared to be highly diagnostic ions allowing the precise location of the acetyl groups to the O‐2 and/or O‐3 of the acetylated galacturonic acid residues. Copyright © 2003 John Wiley & Sons, Ltd.